As there are countless meanings for the auxiliary verb DO, there are multiple ASL hand signs.
My ASL Dictionary features a cartoon of a woman with ginger hair and green eyes illustrating the ASL hand signs for DO, the statement, and the questions.
For a Yes or No question, the Eyebrows go up. Palms up the index finger and thumb of the G hands open and close Twice.
For the question DO WHAT, the Eyebrows furrow down. Palms up the G hands open and close Twice.
The next sign for DO is a lexicalized spelling of DO. Palms up, the index finger of the D hand rapidly taps down on the thumb, closing into an O hand Twice.
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